quantification of mean vessel density in retinoblastoma and its correlation with local tumor invasion and patients survival

background and objectives: retinoblastoma is the most common intraocular pediatric malignancy. angiogenic factor expression such as vegf (vascular endothelial growth factor) in retinoblastoma can be confirmatory angiogenic potential of this tumor. this study was performed to determine the role of angiogenesis in local invasion of retinoblastoma and its correlation with patients’ survival. materials and methods:  this clinicopathological analysis was performed on 60 paraffin- embedded eyes with adequate tumoral tissue, which were stained using a cd34 antibody. microvessel count was carried out in three tumor areas with the richest vascularity (hot spots) at a high magnification (× 400). the obtained data were correlated with histopathological characteristics and 5-years survival. statistical analysis of the data was performed using student t-test and anova test (p